Function analysis / Yeast Two Hybrid Screening
 

Two-hybrid screening (also known as yeast two-hybrid system) is a molecular biology technique to discover intra-cellular protein-protein interactions. We offer such a service using modified Gal4-based system that significantly lowers occurrence of false positives. Our service covers entire screening process from bait construction to the validation of positive clones. 

Key Features:
        Pre-screening analysis: Help customer to select a suitable strategy and library for screening.
        Low false positive rate: Use an improved Gal4-based system.
        High transformation efficiency: Over 1*107 clones will be screened.
        Quality Control: Improve the chance to identify positive interaction.
        Tissue-Specific Libraries: Following libraries are available.
                        ☉Human fetal brain matchmaker cDNA libraries
                        ☉Human lymphocyte matchmaker cDNA libraries
                        ☉Matchmaker HeLa cell cDNA libraries

Service DescriptionTimeline
Phase I. Bait cloning and construction 3 weeks 
Phase II. Toxicity and autoactivation test 2 weeks 
Phase III. Library screening and interaction validation 7-11 weeks 
Total turnaround time 12-16 weeks 
Price $4000/bait gene(for up to 20 positive clones identification) 
 
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