Two-hybrid screening (also known as yeast two-hybrid system) is a molecular biology technique to discover intra-cellular protein-protein interactions. We offer such a service using modified Gal4-based system that significantly lowers occurrence of false positives. Our service covers entire screening process from bait construction to the validation of positive clones.
Key Features:
Pre-screening analysis: Help customer to select a suitable strategy and library for screening.
Low false positive rate: Use an improved Gal4-based system.
High transformation efficiency: Over 1*107 clones will be screened.
Quality Control: Improve the chance to identify positive interaction.
Tissue-Specific Libraries: Following libraries are available.
☉Human fetal brain matchmaker cDNA libraries
☉Human lymphocyte matchmaker cDNA libraries
☉Matchmaker HeLa cell cDNA libraries
| Service Description | Timeline |
| Phase I. Bait cloning and construction | 3 weeks |
| Phase II. Toxicity and autoactivation test | 2 weeks |
| Phase III. Library screening and interaction validation | 7-11 weeks |
| Total turnaround time | 12-16 weeks |
| Price | $4000/bait gene(for up to 20 positive clones identification) |
|