MAb production is divided into different phases.
The customer has the option to be invoiced for each phase at its initiation.
| Phase I: Immunization(5 mice/antigen)& Sera Testing by ELISA | $700 |
| Phase II: Fusion and Colony Screening | $1800 |
| Phase III: Subcloning, Colony Expansion & Frozen | $350 |
| Phase IV: Isotyping and Ascites/serum free Production (2 clones) | $350 |
| Phase V: Antibody Purification by Protein A/G (2 clones, 3mg each) | $350 |
| Total Price (Phase I-C V) | $3550 |
| Charge for each additional hybridoma | $350 |
| Charge for each additional antibody production | $350 |
The customer can provide a ready-to-inject antigen or peptide antigen for MAb production.
When using a peptide supplied by the customer, additional charge for KLH conjugation is $200.
| In-Vivo Production | Price per mouse |
| Ascites Production from 1-25 Mice | $25.00 |
| Ascites Production from 26-50 Mice | $24.50 |
| Ascites Production from 51-100 Mice | $24.00 |
| Ascites Production from 101-150 Mice | $23.50 |
| Ascites Production from 151-200 Mice | $23.00 |
| Ascites Production from 200+ Mice | $19 - $22.50 |
| Service Type | Service Content | Price |
Basic Service |
Using a ready to inject antigen (2mg) supplied by the customer. Service includes two New Zealand White Rabbits immunization, prebleed, blood collection (40~60ml) and titration. Approx. time required: 3 months | $790/antigen |
| Standard Service | Using a peptide (2mg) supplied by the customer.Service includes peptide conjugation to KLH, two New Zealand White Rabbits immunization, prebleed, blood collection (40~60ml) and titration. Approx. time required: 3 months |
$920/antigen |
| Optional Service | Antibody purification by peptide affinity column (peptide supplied by customer, antiserum from 2 rabbits) | $450/peptide |
| Antibody purification by protein A/G | $200/antigen |
SG could also provide peptide synthesis service which is $16/amono acid.
We also provide additional services to evaluate the application of antibodies on Immunoprecipitation,
Western blotting, and Immunohistochemistry on normal and disease tissues.
| Validation Type | Service Content | Price |
| Western analysis (antigen overexpression in mammalian cells) | Transient transfection of the target cDNA (provided by customer) into mammalian cells and Western-blot with the antibody. | $400 |
| Western analysis (endogenous detection in a mammalian cell line) | The customer will choose the cell line or provide the specific cells. | $400 |
| Immunoprecipitation analysis (based on antigen overexpression in mammalian cells) | Transient transfection of flag -tagged target cDNA (original target cDNA provided by customer) into mammalian cells, immunoprecipitated with target antibody and Western-blot detection with anti-flag. | $600 |
| Immunohistochemistry analysis | Using a paraffin-embedded normal or tumor tissue selected by the customer: antibody's specification and sensitivity (up to 6 tissue samples). | $120/tissue |
Monoclonal antibody against human G1 protein for western blot use
Customer request: To generate monoclonal antibody specifically against human G1 protein which can
be applied in western blot detection, no cross reaction with the other family member proteins (G2 and
G3 protein), but could react with mouse G1 protein.
Polyclonal antibody against human H protein for western blot and immuno-precipitation use
Customer request: To generate polyclonal antibody against human H protein that can be applied in both western
blot detection and immuno-precipitation.
Contact Info:
Ms. Wei Zhang
Project Management
Office: +86-21-51319016
+86-21-50802786 Ext: 188
Fax: +86-21-50802783
Email: service@shanghaigenomics.com
Service Web:
www.genomicservice.com
Shanghai Genomics, Inc.647 Song Tao Road, Building 1,Zhangjiang Hi-Tech Park
Shanghai 201203,P.R.China