SG has successfully established gene disruption platforms including siRNA and microRNA knockdowns. Using siRNA technology, SG has validated effective probes for the whole human kinome.

Gene disruption is a powerful method in biomedical researches. Because of the low frequency of homological recombination, it is quite difficult to disrupt genes by direct genetic modification. Therefore, transcriptional knockdown by RNAi becomes popular in a large number of R&D areas. Conditional RNAi is one of the best choices for this purpose. SG has an experienced group in both RNAi techology (shRNA and miR-shRNA) and stable cell line development. In addition, SG owns a cell library, in which many ordinary ATCC sourced cell lines are available for use. In combination with our efficient DNA subcloning techniques, we are able to provide a more customized service.

We offer:

1)     Targeting probe design, construction and validation

2)     Transiently targeting

3)     Long-term targeting in either poly-or mono-clonal stable cell line