Shanghai Genomics (SG) has successfully established gene disruption platforms including siRNA and microRNA knockdowns. Using siRNA technology, SG has validated effective probes for the entire human kinome.

Shanghai Genomics (SG) has a broad experience in identification of effective miR-shRNA probes for genes of interest. In addition, SG has set up a convenient way to switch shRNA to miR-shRNA so that we can provide more flexible services to its clients.

Services Include:

1)     miR-shRNA Design and Vector Construction

2)     shRNA to miR-shRNA Transfer

3)     miR-shRNA Probe Validation

4)     Knockdown of Selected Genes (Transient or Long-Term)

Endogenous miRNA has attracted a lot attention because of its critical role in the regulation of gene expression during embryonic development. The design of shRNA transcripts, as primary microRNA (miR) mimics (miR-shRNAs) driven by Pol II promoters which significantly expands the possibilities for conditional RNAi in mammalian cells. This technology minimizes the problems caused by long-term gene suppression from Pol II-shRNA; implying an ideal application for gene therapy with a controllable gene knockdown.