SG has successfully established gene disruption platforms including siRNA and microRNA knockdowns. Using siRNA technology, SG has validated effective probes for the whole human kinome.

Endogenous miRNA has attracted a lot attention because of its critical role in regulation of gene expression during embryonic development. The design of shRNA transcripts as primary microRNA (miR) mimics (miR-shRNAs) driven by pol II promoters significantly expands the possibilities for conditional RNAi in mammalian cells. This technology minimizes the problems caused by long-term gene suppression from pol II-shRNA, implying an ideal application for gene therapy with a regulatable gene knockdown. Shanghai Genomics (SG) has a broad experience in identification of effective miR-shRNA probes for genes of interest. In addition, SG has set up a convenient way to switch shRNA to miR-shRNA so that we can provide a more flexible service to its customers.

We offer:

1)     miR-shRNA design and miR-shRNA vector construction

2)     Transfer shRNA to miR-shRNA

3)     miR-shRNA probe validation

4)     Transient or long-term knockdown of selected genes