SG has successfully established gene disruption platforms including siRNA and microRNA knockdowns. Using siRNA technology, SG has validated effective probes for the whole human kinome.

As one of the most powerful RNA interference (RNAi) tools, short hairpin RNA (shRNA) technology has been widely used in biomedical research. When expressed in mammalian cells, the shRNA can be converted into small interfering RNA (siRNA). These siRNA molecules, initiate cleavage and degradation of specific mRNA molecules, providing either transient or long-term target gene suppression.

Shanghai Genomics (SG) has successfully set up an efficient and high throughput platform to validate effective shRNAs for genes of interest. With high quality real-time PCR analysis, SG has successfully validated more than 2,800 shRNA probes for whole human kinome. SG’s broad experience and the maturity of our experimental shRNA platform ensure that we can provide fast and high quality RNAi services.

We offer:

1)     shRNA design and shRNA vector construction

2)     shRNA probe validation

3)     Transient or long-term knockdown of selected genes